EEF2 Kit ELISA

N° du produit ABIN7830613

Kit ELISA EEF2 Humain, Colorimetric test pour la quantification de Humain EEF2.

Aperçu rapide pour EEF2 Kit ELISA (ABIN7830613)

Antigène: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit EEF2 Kit ELISA

Fonction: EEF2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EEF2 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Autre désignation: EEF2

Sujet:

Synonyms: EEF-2|EF2

Gene Description: eukaryotic translation elongation factor 2

ID gène: 1938

UniProt: P13639

Pathways: AMPK Signaling