PKC mu Kit ELISA

N° du produit ABIN7830857

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain PKC mu.

Aperçu rapide pour PKC mu Kit ELISA (ABIN7830857)

Antigène: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit PKC mu Kit ELISA

Fonction: PRKD1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKD1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Autre désignation: PRKD1

Sujet:

Synonyms: PKC-MU|PKCM|PKD|PRKCM

Gene Description: protein kinase D1

ID gène: 5587

Pathways: Myometrial Relaxation and Contraction