PIKFYVE Kit ELISA

N° du produit ABIN7830968

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain PIKFYVE.

Aperçu rapide pour PIKFYVE Kit ELISA (ABIN7830968)

Antigène: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Détail du produit PIKFYVE Kit ELISA

Fonction: PIP5K3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PIP5K3 expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Autre désignation: PIP5K3

Sujet:

Synonyms: CFD|FAB1|KIAA0981|MGC40423|PIKFYVE|PIP5K

Gene Description: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III

ID gène: 200576

Pathways: Inositol Metabolic Process