NME2 Kit ELISA

N° du produit ABIN7830985

Kit ELISA NME2 Humain, Colorimetric test pour la quantification de Humain NME2.

Aperçu rapide pour NME2 Kit ELISA (ABIN7830985)

Antigène: NME2 (Nucleoside diphosphate kinase B (NME2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit NME2 Kit ELISA

Fonction: NME2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NM23 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 4°C for six months.

Détail du antigène

Antigène: NME2 (Nucleoside diphosphate kinase B (NME2))

Autre désignation: NME2

Sujet:

Synonyms: MGC111212|NDPK-B|NDPKB|NM23-H2|NM23B|puf

Gene Description: non-metastatic cells 2, protein (NM23B) expressed in

ID gène: 4831

UniProt: P22392