NCF1 Kit ELISA

N° du produit ABIN7830989

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain NCF1.

Aperçu rapide pour NCF1 Kit ELISA (ABIN7830989)

Antigène: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit NCF1 Kit ELISA

Fonction: NCF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of p47 phox expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 4°C for six months.

Détail du antigène

Antigène: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Autre désignation: NCF1

Sujet:

Synonyms: FLJ79451|NCF1A|NOXO2|SH3PXD1A|p47phox

Gene Description: neutrophil cytosolic factor 1

ID gène: 653361

UniProt: P14598

Pathways: Signalisation PI3K-Akt