PDE4C Kit ELISA

N° du produit ABIN7830993

Le kit ELISA PDE4C (ABIN7830993) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain PDE4C.

Aperçu rapide pour PDE4C Kit ELISA (ABIN7830993)

Antigène: PDE4C (phosphodiesterase 4C, CAMP-Specific (PDE4C))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit PDE4C Kit ELISA

Fonction: PDE4D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PDE4D expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 4°C for six months.

Détail du antigène

Antigène: PDE4C (phosphodiesterase 4C, CAMP-Specific (PDE4C))

Autre désignation: PDE4C

Sujet:

Synonyms: DPDE1|MGC126222

Gene Description: phosphodiesterase 4C, cAMP-specific (phosphodiesterase E1 dunce homolog, Drosophila)

ID gène: 5143

UniProt: Q08493

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process