TUBA1A Kit ELISA

N° du produit ABIN7831010

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain TUBA1A.

Aperçu rapide pour TUBA1A Kit ELISA (ABIN7831010)

Antigène: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit TUBA1A Kit ELISA

Fonction: TUBA1A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Tubulin alpha expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 4°C for six months.

Détail du antigène

Antigène: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Autre désignation: TUBA1A

Sujet:

Synonyms: B-ALPHA-1|FLJ25113|LIS3|TUBA3

Gene Description: tubulin, alpha 1a

ID gène: 7846

UniProt: Q71U36

Pathways: Dynamique des Microtubules, M Phase