NDC80 Kit ELISA

N° du produit ABIN7831026

Le kit ELISA NDC80 (ABIN7831026) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain NDC80.

Aperçu rapide pour NDC80 Kit ELISA (ABIN7831026)

Antigène: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit NDC80 Kit ELISA

Fonction: NDC80 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NDC80 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 4°C for six months.

Détail du antigène

Antigène: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Autre désignation: NDC80

Sujet:

Synonyms: HEC|HEC1|KNTC2|TID3|hsNDC80

Gene Description: NDC80 homolog, kinetochore complex component (S. cerevisiae)

ID gène: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location