25-OH Vitamin D Kit ELISA

N° du produit ABIN7831163

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain 25-OH Vitamin D.

Aperçu rapide pour 25-OH Vitamin D Kit ELISA (ABIN7831163)

Antigène: 25-OH Vitamin D (25-OH-D)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Classe de qualité: QuickTest

Détail du produit 25-OH Vitamin D Kit ELISA

Fonction: FastELISA 25-OH-D (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of Human 25-OH-D (25-Hydroxyvitamin D) in Serum, plasma.

Analytical Method: Quantitative

Specificité: This assay is highly specific for detection of Human 25-OH-D. No significant cross-reactivity or interference between Human 25-OH-D and analogues was observed.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Date de péremption: 6 months

Détail du antigène

Antigène: 25-OH Vitamin D (25-OH-D)

Autre désignation: 25-OH-D

Classe de substances: Chemical