IgG3 Kit ELISA

N° du produit ABIN7832844

Le kit ELISA IgG3 (ABIN7832844) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris IgG3.

Aperçu rapide pour IgG3 Kit ELISA (ABIN7832844)

Antigène: IgG3

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Classe de qualité: QuickTest

Détail du produit IgG3 Kit ELISA

Fonction: FastELISA IgG3 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Mouse IgG3 (Immunoglobulin G3) in serum, plasma.

Analytical Method: Quantitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C

Stockage commentaire: Store at 2-8C for 6 months.Avoid repeated freezing and thawing.

Date de péremption: 6 months

Détail du antigène

Antigène: IgG3

Classe de substances: Antibody