CST3 Kit ELISA
Aperçu rapide pour CST3 Kit ELISA (ABIN7832858)
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Voir toutes CST3 Kits ELISAReactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
Classe de qualité
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Fonction
- FastELISA CST3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human CST3 (Cystatin C) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificité
- This assay is highly specific for detection of Human CST3. No significant cross-reactivity or interference between Human CST3 and analogues was observed.
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Cystatin C (CST3) ELISA Kit
CST3 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Souris Colorimetric Sandwich ELISA 2.5-160 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Humain Colorimetric Sandwich ELISA 7.8-500 ng/mL Urine
Cystatin C (CST3) ELISA KitCST3 Reactivité: Souris Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Urine
Cystatin C (CST3) ELISA KitCST3 Reactivité: Porc Colorimetric Sandwich ELISA 125 ng/mL - 8000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Singe Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Boeuf (Vache) Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivité: Humain AA 1-146 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Pre-coated
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Protocole
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- Avoid repeated freezing and thawing
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Date de péremption
- 6 months
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- CST3 (Cystatin C (CST3))
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Autre désignation
- CST3
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Sujet
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Synonyms: ARMD11|MGC117328
Gene Description: cystatin C
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ID gène
- 1471
Antigène Voir toutes CST3 Kits ELISA
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