Osteocalcin Kit ELISA
Aperçu rapide pour Osteocalcin Kit ELISA (ABIN7832874)
Antigène
Voir toutes Osteocalcin (BGLAP) Kits ELISAReactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
Classe de qualité
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Fonction
- FastELISA OC (Huamn) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human OC (Osteocalcin) in Serum.
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Analytical Method
- Quantitative
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Specificité
- This assay is highly specific for detection of Human OC. No significant cross-reactivity or interference between Human OC and analogues was observed.
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Osteocalcin (BGLAP) ELISA Kit
BGLAP Reactivité: Humain Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Souris Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Poulet Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Lapin Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Porc Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Rat Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Osteocalcin (BGLAP) ELISA KitBGLAP Reactivité: Humain Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Pre-coated
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Protocole
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- Avoid repeated freezing and thawing
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Date de péremption
- 6 months
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- Osteocalcin (BGLAP)
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Autre désignation
- BGLAP
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Sujet
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Synonyms: BGP|OC|PMF1
Gene Description: bone gamma-carboxyglutamate (gla) protein
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ID gène
- 632
Antigène Voir toutes Osteocalcin (BGLAP) Kits ELISA
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