alpha Fetoprotein Kit ELISA

N° du produit ABIN7832945

Le kit ELISA alpha Fetoprotein (ABIN7832945) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain alpha Fetoprotein.

Aperçu rapide pour alpha Fetoprotein Kit ELISA (ABIN7832945)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 781 pg/mL - 50000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit alpha Fetoprotein Kit ELISA

Seuil minimal de détection: 781 pg/mL

Fonction: Sandwich ELISA for quantitative detection of Human Alpha-fetoglobulin concentrations.

Analytical Method: Quantitative

Sensibilité: 280 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: AFP

Sujet: Alternative Names: HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

ID gène: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process