FBLN3 Kit ELISA

N° du produit ABIN7833082

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris FBLN3.

Aperçu rapide pour FBLN3 Kit ELISA (ABIN7833082)

Antigène: FBLN3 (Fibulin 3 (FBLN3))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit FBLN3 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich ELISA for quantitative detection of Mouse Fibulin 3 concentrations.

Analytical Method: Quantitative

Sensibilité: 7 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FBLN3 (Fibulin 3 (FBLN3))

Autre désignation: EFEMP1

Sujet: Alternative Names: FBLN3, FBNL, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3

UniProt: Q8BPB5

Pathways: EGFR Signaling Pathway