CCL17 Kit ELISA
Aperçu rapide pour CCL17 Kit ELISA (ABIN7849826)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 31.2 pg/mL
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Fonction
- For quantitative detection of CCL17,TARC in mouse serum, plasma, tissue lysatesor cell culture supernatants.
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Analytical Method
- Quantitative
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Sensibilité
- < 7 pg/mL
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Matériel non inclus
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- 37 °C incubator
- Microplate reader (wavelength: 450 nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5 mL of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1 L
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Plaque
- Pre-coated
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Préparation des réactifs
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store at 2-8°C for 6 months, or at -20°C for 12 months.
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Date de péremption
- 12 months
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- CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))
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Autre désignation
- CCL17,TARC
Antigène Voir toutes CCL17 Kits ELISA
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