REG3A Kit ELISA

N° du produit ABIN8055500

Kit ELISA REG3A Souris, Colorimetric test pour la quantification de Souris REG3A.

Aperçu rapide pour REG3A Kit ELISA (ABIN8055500)

Antigène: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Épitope: AA 27-175

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Détail du produit REG3A Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Mouse REG3A ELISA Kit to quantitate Mouse Reg3a in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: E27-Q175

Capture antibody and Detection antibody: A monoclonal antibody from rat|a biotinylated detection polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.3%, 5.2%, 4.7%
Inter-Assay Precision (CV%): 6.4%, 5.6%, 4.4%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Détail du antigène

Antigène: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Autre désignation: Reg3a

Sujet:

Background: Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3, 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known.

Gene Full Name: regenerating islet-derived 3 alpha

ID gène: 19694

UniProt: O09037