SCGB3A1 Kit ELISA

N° du produit ABIN8055566

Kit ELISA SCGB3A1 Rat, Colorimetric test pour la quantification de Rat SCGB3A1.

Aperçu rapide pour SCGB3A1 Kit ELISA (ABIN8055566)

Antigène: SCGB3A1 (Secretoglobin, Family 3A, Member 1 (SCGB3A1))

Épitope: AA 22-104

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit SCGB3A1 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Rat SCGB3A1 ELISA Kit to quantitate Rat SCGB3A1 in cell culture supernatants, serum and plasma (heparin or EDTA) .

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: F22-G104

Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.7%, 5.2%, 5.7%
Inter-Assay Precision (CV%): 6.3%, 5.4%, 7.1%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Détail du antigène

Antigène: SCGB3A1 (Secretoglobin, Family 3A, Member 1 (SCGB3A1))

Autre désignation: SCGB3A1

Sujet:

Background: Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. It is mapped to 10q21. Members of the secretoglobin family, such as SCGB3A1, are secreted proteins of about 10 kD that are found in high concentrations in fluids of lung, lacrimal gland, salivary gland, prostate, uterus, and other tissues.

Gene Full Name: secretoglobin, family 3A, member 1

ID gène: 338418

UniProt: E9PU36