CRP Kit ELISA

N° du produit ABIN8057098

Le kit ELISA CRP (ABIN8057098) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat CRP.

Aperçu rapide pour CRP Kit ELISA (ABIN8057098)

Antigène: CRP (C-Reactive Protein (CRP))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.13 pg/mL - 3000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit CRP Kit ELISA

Seuil minimal de détection: 3.13 pg/mL

Analytical Method: Quantitative

Specificité: Hs-CRP

Ingrédients:

• Plate
• Standard
• Diluent

Matériel non inclus: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat CRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: protect your eyes

Stock: 4 °C

Stockage commentaire: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Détail du antigène

Antigène: CRP (C-Reactive Protein (CRP))

Autre désignation: hs-CRP

Sujet: C-reactive protein (CRP) is an annular, pentameric protein. The physiological role of CRP is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex. CRP is an acute phase protein in humans and is an important mediator of immune host defense. The acute phase response occurs in response to a wide range of acute and chronic inflammatory conditions such as bacterial, viral, or fungal infections, rheumatic and other inflammatory diseases, malignancy, and tissue injury and necrosis. These conditions cause release of IL-6 and other cytokines that trigger the synthesis of CRP and fibrinogen by the liver. CRP plays a role in innate immunity as an early defense system against infections. Monitoring of CRP levels in serum provides more detailed insights in several pathological situations such as inflammatory disease (including inflammatory bowel disease, Intestinal Lymphoma, Intestinal Tuberculosis and Behcet's Syndrome), cancer, diabetes, hypertension, cardiovascular disease, obstructive sleep apnea and so on.

ID gène: 25419

NCBI Accession: NM_017096

UniProt: P48199

Pathways: Carbohydrate Homeostasis