CA242 Kit ELISA

N° du produit ABIN8065412

Kit ELISA CA242 Humain, Colorimetric test pour la quantification de Humain CA242.

Aperçu rapide pour CA242 Kit ELISA (ABIN8065412)

Antigène: CA242 (Carbohydrate Antigen 242 (CA242))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL – 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Détail du produit CA242 Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Fonction: In vitro quantitative determination of ca242 concentrations in serum, plasma, cell culture supernatant

Analytical Method: Quantitative

Specificité: Specifically recognize ca242, no obvious cross reaction with other analogues

Sensibilité: 18.75 pg/mL

Ingrédients: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Matériel non inclus: - Microplate reader (wavelength: 450nm)
- 37 °C incubator
- Automated plate washer
- Precision single and multi-channel pipette and disposable tips
- Clean tubes and Eppendorf tubes
- Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Volume d'échantillon: 100 μL

Durée du test: 4 h

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ca242 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ca242 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ca242 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ca242 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Précision du teste: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate. CV% = <6%
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.CV%= <7%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Détail du antigène

Antigène: CA242 (Carbohydrate Antigen 242 (CA242))

Autre désignation: CA242

Sujet: CA242(Pancreatic Carcinoma Markers-CA242)