5-Hydroxymethylcytosine Kit ELISA

N° du produit ABIN8067734

Kit ELISA 5-Hydroxymethylcytosine Boeuf (Vache), Cheval, Humain, Souris, Porc, Rat et Mouton, Colorimetric test pour la quantification de Boeuf (Vache), Cheval, Humain, Souris, Porc, Rat et Mouton 5-Hydroxymethylcytosine.

Aperçu rapide pour 5-Hydroxymethylcytosine Kit ELISA (ABIN8067734)

Antigène: 5-Hydroxymethylcytosine (5-hmC)

Reactivité: Boeuf (Vache), Cheval, Humain, Souris, Porc, Rat, Mouton

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 pg/mL - 100000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Urine

Détail du produit 5-Hydroxymethylcytosine Kit ELISA

Seuil minimal de détection: 0.1 pg/mL

Fonction: 5-hmC ELISA Kit is an in vitro quantitative assay for detecting 5-Hydroxymethylation based on the competitive enzyme immunoassay principle.

Analytical Method: Quantitative

Specificité: This EIA kit detects 5-hmC / 5-Hydroxymethylcytosine

Sensibilité: 1.006 pg/mL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Lyophilized Standard
• Capture Antibody
• Assay Diluent
• Biotinylated Peptide
• HRP-Streptavidin
• Positive Control
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Software which can perform four-parameter logistic regression models
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Aluminum foil
• Plastic wrap

Information d'application

Indications d'application: Suggested Plasma Dilution: 2-4 folds

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL Capture Antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
3. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
4. Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: 5-Hydroxymethylcytosine (5-hmC)

Autre désignation: 5-hmC

Classe de substances: Chemical

Sujet: Optional[Gene Symbols]: 5-hydroxymethylcytosine (5-hmC), also known as the ""sixth base"" of DNA, is generated by the oxidation of 5-methylcytosine (5-mC) through the action of the ten-eleven translocation (TET) family of enzymes. 5-hmC is thought to be the DNA demethylation pathway, thereby playing a crucial part in gene regulation, chromatin structure and accessibility, recruiting or repelling transcription factors, and ultimately modulating gene expression. The presence of 5-hmC is particularly abundant in neuronal cells, suggesting a significant role in brain development and function. In addition, levels of 5-hmC undergo highly dynamic changes during development, differentiation and cancer. 5-hmC plays a critical role in developmental processes and dysregulation of 5-hmC has been associated with several human diseases.