Cadherin 5 Kit ELISA

N° du produit ABIN8068126

Le kit ELISA Cadherin 5 (ABIN8068126) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Cadherin 5.

Aperçu rapide pour Cadherin 5 Kit ELISA (ABIN8068126)

Antigène: Cadherin 5 (CDH5)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.66 ng/mL - 500 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit Cadherin 5 Kit ELISA

Seuil minimal de détection: 0.66 ng/mL

Fonction: Human VE-Cadherin (CDH5) ELISA Kit for Cell Culture Supernatants and Serum samples.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALCAM (CD166), BCAM, CEACAM-1, E-Cadherin, TROP1 (EpCAM), E-Selectin, ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), L-Selectin (CD62L), NCAM-1 (CD56), NrCAM, P-Cadherin, P-Selectin, PECAM-1 (CD31), VCAM-1 (CD106).

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: Cadherin 5 (CDH5)

Autre désignation: VE-Cadherin

Sujet:

Gene Symbols: CDH5

Protein Name (Synonyms): Cadherin-5 (7B4 antigen) (Vascular endothelial cadherin) (VE-cadherin) (CD antigen CD144)

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2