Corticosterone Kit ELISA

N° du produit ABIN8068521

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain, Lapin, Porc, Chien, Mouton, Cat et Cheval Corticosterone.

Aperçu rapide pour Corticosterone Kit ELISA (ABIN8068521)

Antigène: Corticosterone (CORT)

Reactivité: Humain, Lapin, Porc, Chien, Mouton, Cat, Cheval

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.0001 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Saliva, Serum, Urine

Détail du produit Corticosterone Kit ELISA

Seuil minimal de détection: 0.0001 ng/mL

Fonction: This kit is a competitive immunoassay that quantifies corticosterone in serum or plasma from human, porcine, feline, canine, rabbit, equine, and sheep samples (not rat or mouse) within 2.5 hours.

Analytical Method: Quantitative

Specificité: This EIA kit detects Corticosterone

Sensibilité: 0.0021 ng/mL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Information d'application

Indications d'application: Suggested Plasma Dilution: 2-4 folds

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Corticosterone (CORT)

Autre désignation: Corticosterone

Classe de substances: Hormone

Sujet: Optional[Gene Symbols]: Corticosterone, a glucocorticoid synthesized in the adrenal cortex upon ACTH stimulation, serves as both the biosynthetic precursor of aldosterone and a key biomarker of stress. Stress-induced elevations in corticosterone have been linked to impaired long-term memory retrieval, chronic increases following dietary restriction, and marked rises after burn injury. Beyond its role in the stress response, corticosterone also helps shape sleep-wake cycles.

The Corticosterone Enzyme Immunoassay (EIA) Kit (EIA-CORT2) is a competitive immunoassay that quantifies corticosterone in serum or plasma from human, porcine, feline, canine, rabbit, equine, and sheep samples (not rat or mouse) within 2.5 hours. Absorbance is measured at 450 nm and values are interpolated from a ready-to-use corticosterone standard curve. The assay demonstrates a sensitivity of 0.0021 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.