CRH Kit ELISA

N° du produit ABIN8068526

Kit ELISA CRH Souris, Humain et Rat, Colorimetric test pour la quantification de Souris, Humain et Rat CRH.

Aperçu rapide pour CRH Kit ELISA (ABIN8068526)

Antigène: CRH (Corticotropin Releasing Hormone (CRH))

Reactivité: Souris, Humain, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.016 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit CRH Kit ELISA

Seuil minimal de détection: 0.016 ng/mL

Fonction: Competitive ELISA (EIA) kit for detecting Corticotropin Releasing Factor (CRF), also known as Corticotropin Releasing Hormone (CRH), in serum and cell culture medium samples.

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Corticotropin Releasing Factor

Sensibilité: 0.1 ng/mL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Suggested Plasma Dilution: Human: 4X / Mouse: 2X / Rat: 2X

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards as instructed.
• Add 100 μL detection antibody to each well.
• Incubate 1.5 h at RT or O/N at 4 °C.
• Add 100 μL standard or sample to each well.
• Incubate 2.5 h at RT.
• Add 100 μL prepared streptavidin solution.
• Incubate 45 min at RT.
• Add 100 μL TMB One-Step Substrate Reagent to each well.
• Incubate 30 min at RT.
• Add 50 μL Stop Solution to each well.
• Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, Biotinylated CRF / CRH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Détail du antigène

Antigène: CRH (Corticotropin Releasing Hormone (CRH))

Autre désignation: Corticotropin Releasing Factor

Sujet:

Gene Symbols: CRH

Background: Corticotropin-releasing factor (CRF) (also known as Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Its main function is the stimulation of the pituitary synthesis of adrenocorticotropic hormone (ACTH), as part of the hypothalamic-pituitary-adrenal axis (HPA axis). CRF levels may be associated with Alzheimer"s disease and major depression. CRF is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta, suggesting roles in parturition.

The RayBiotech CRF Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting CRF peptide based on the competitive enzyme immunoassay principle. In this assay, a biotinylated CRF peptide is spiked into the samples and standards. The samples and standards are then added to the plate, where the biotinylated CRF peptide competes with endogenous (unlabeled) CRF for binding to the anti-CRF antibody. After a wash step, any bound biotinylated CRF then interacts with horseradish peroxidase (HRP)-streptavidin, which catalyzes a color development reaction. The intensity of the colorimetric signal is directly proportional to the amount of captured biotinylated CRF peptide and inversely proportional to the amount of endogenous CRF in the standard or samples. A standard curve of known concentration of CRF peptide can be established and the concentration of CRF peptide in the samples can be calculated accordingly.

Protein Name (Synonyms): Corticotropin-releasing hormone (CRH), corticotropin-releasing factor (CRF), corticoliberin

ID gène: 1392

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour