Cortisol Kit ELISA

N° du produit ABIN8068527

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain, Souris et Rat Cortisol.

Aperçu rapide pour Cortisol Kit ELISA (ABIN8068527)

Antigène: Cortisol

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Cortisol Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: Human/Mouse/Rat Cortisol EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Cortisol.

Sensibilité: 2.7 ng/mL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Standard Peptide
• HRP-Conjugated Peptide
• Capture Antibody
• Positive Control
• Wash Buffer
• Assay Diluent(s)
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Distilled or deionized water
• Aluminum foil
• Plastic wrap

Information d'application

Indications d'application: Suggested Plasma Dilution: Human: 8x / Mouse: 2x / Rat: 2x

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
3. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Cortisol

Classe de substances: Hormone

Sujet: Optional[Gene Symbols]: Cortisol is a naturally occurring pregnane corticosteroid and is also known as 11β,17α,21-trihydroxypregn-4-ene-3,20-dione. Cortisol is synthesized from cholesterol mainlyby the adrenal gland in the zona fasciculata. Release of Cortisolis increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation.