DENV IgM Kit ELISA

N° du produit ABIN8068630

Le kit ELISA DENV IgM (ABIN8068630) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain DENV IgM.

Aperçu rapide pour DENV IgM Kit ELISA (ABIN8068630)

Antigène: DENV IgM (Dengue Virus Antibody IgM (DENV IgM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Gamme de detection: 1.37 units/mL - 1000 units/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit DENV IgM Kit ELISA

Seuil minimal de détection: 1.37 units/mL

Fonction: Dengue Virus 1 E Protein Human IgM ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgM antibody against Dengue Virus 1 Envelope Protein (E) in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Analytical Method: Semi-Quantitative

Ingrédients:

Size / Description
• Pathogen antigen coated 96-well Microplate
• 96 wells (12 strips x 8 wells) coated with pathogen antigen
• Wash Buffer Concentrate (20X)
• 25 mL of 20X concentrated solution
• Positive Control
• 1 vial of Positive Control sample containing anti-pathogen antigen antibody
• Biotinylated Anti-Human Ig(A/G/M)
• 1 vial of solution
• Biotinylated Anti-Positive Control
• 1 vial of solution
• HRP-Streptavidin concentrate
• 1 vial of solution
• TMB One-Step Substrate Reagent
• 12 mL of 3,3,5,5'-tetramethylbenzidine (TMB buffer solution)
• Stop Solution
• 8 mL of 0.2 M sulfuric acid
• Assay Diluent B
• 15 mL of 5X concentrated buffer
• 5X Sample Diluent
• 25 mL of 5X diluent buffer, 0.5% proclin 300 as preservative

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Shaker
• Pipettes capable of accurately delivering 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Graduated cylinders: 100 mL and 1 liter
• Distilled or deionized water
• Absorbent paper

Information d'application

Indications d'application: Suggested Plasma Dilution: 1000-fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Positive Control and Biotinylated Anti-Human Ig(A/G/M) Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Détail du antigène

Antigène: DENV IgM (Dengue Virus Antibody IgM (DENV IgM))

Autre désignation: Dengue Virus IgM

Classe de substances: Antibody

Sujet:

Gene Symbols: IgM

Background: This in-vitro ELISA kit employs an indirect ELISA method. Standard 96-well plates are coated with the pathogen antigen, which combines with the corresponding antibody present in a sample and positive control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA/G/M antibody is added, a complex forms between the biotinylated secondary antibody and human antibody bound to the protein-coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The Positive Control consists of an anti-pathogen antigen antibody. A biotinylated anti-positive control antibody is added to the positive control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.

Protein Name (Synonyms): IgM, Dengue Virus 1 Envelope Protein (E)