Digoxin Kit ELISA

N° du produit ABIN8068667

Kit ELISA Digoxin Humain, Boeuf (Vache) et Souris, Colorimetric test pour la quantification de Humain, Boeuf (Vache) et Souris Digoxin.

Aperçu rapide pour Digoxin Kit ELISA (ABIN8068667)

Antigène: Digoxin (DIG)

Reactivité: Humain, Boeuf (Vache), Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.01 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Saliva, Serum, Urine

Détail du produit Digoxin Kit ELISA

Seuil minimal de détection: 0.01 ng/mL

Fonction: This kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours.

Analytical Method: Quantitative

Specificité: This EIA kit detects Digoxin

Sensibilité: 0.0427 ng/mL

Ingrédients:

Size / Description
• DIG Microplate
• 96 wells coated with DIG
• Assay Diluent
• Standard DIG
• Positive Control
• Anti-DIG Antibody
• ~10 μL
• HRP-conjugated Secondary Antibody
• ~5 μL
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• GraphPad Prism or SigmaPlot software

Information d'application

Indications d'application: Suggested Plasma Dilution: 2-4 folds

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL anti-DIG to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10-25 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Digoxin (DIG)

Autre désignation: Digoxin

Classe de substances: Chemical

Sujet: Optional[Gene Symbols]: Digoxin (DIG) is a once-daily medication used to treat certain heart conditions when first-line therapies are ineffective or when additional treatment is required. Because digoxin has a narrow therapeutic index, careful monitoring is essential. Excessive digoxin levels can lead to dangerous, and sometimes fatal, cardiac arrhythmias. Therefore, serum digoxin testing helps ensure that drug levels are sufficient to provide therapeutic benefit while avoiding toxicity.

The DIG Enzyme Immunoassay (EIA) Kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours. Absorbance is measured at 450 nm, and concentrations are interpolated from a ready-to-use DIG standard curve. The assay demonstrates a sensitivity of 0.0427 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.