Estradiol Kit ELISA

N° du produit ABIN8068770

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain, Mouton, Chien, Cheval, Porc, Lapin et Cat Estradiol.

Aperçu rapide pour Estradiol Kit ELISA (ABIN8068770)

Antigène: Estradiol

Reactivité: Humain, Mouton, Chien, Cheval, Porc, Lapin, Cat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.01 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Urine

Détail du produit Estradiol Kit ELISA

Seuil minimal de détection: 0.01 pg/mL

Fonction: Estradiol Enzyme Immunoassay Kit is a competitive enzyme immunoassay developed for rapid E2 detection within 2.5 hours in serum and plasma samples collected from various species.

Analytical Method: Quantitative

Sensibilité: 0.09 pg/mL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Information d'application

Indications d'application: Suggested Plasma Dilution: 2-3 folds

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Estradiol

Classe de substances: Hormone

Sujet: Optional[Gene Symbols]: Estradiol (E2) is an estrogen steroid hormone, also known as 17β-estradiol (to distinguish it from 17α-estradiol) or as estra-1,3,5(10)-triene-3,17β-diol. It is the major female sex hormone, and involved in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is responsible for the female sexual development, and is important in the development and maintenance of female reproductive tissues. Estradiol is produced mainly by the granulosa cells of the ovaries, is also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain.