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IgA Kit ELISA

Kit ELISA IgA Humain, Colorimetric test pour la quantification de Humain IgA.
N° du produit ABIN8069165
1.942,21 €
Frais d'envoi 40,00 €, glace carbonique 20,00 € et TVA exclus
5 x 96 tests
Destination: France
Envoi sous 6 à 9 jours ouvrables

Aperçu rapide pour IgA Kit ELISA (ABIN8069165)

Antigène

Voir toutes IgA Kits ELISA
IgA

Reactivité

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Humain

Méthode de détection

Colorimetric

Type de méthode

Indirect ELISA

Application

ELISA

Type d'échantillon

Dried Blood, Plasma, Serum
  • Fonction

    COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgA antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

    Analytical Method

    Semi-Quantitative

    Ingrédients

    • SARS-CoV-2 N-protein coated 96-well microplate
    • Wash Buffer
    • Positive Control
    • Negative Control
    • HRP-anti positive control IgA Antibody
    • HRP-anti Human IgA Antibody
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diluent
    ,
    • SARS-CoV-2 N-protein coated 96-well microplate
    • Wash Buffer
    • Positive Control
    • Negative Control
    • HRP-anti positive control IgA Antibody
    • HRP-anti Human IgA Antibody
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diluent

    Matériel non inclus

    • Microplate reader capable of measuring absorbance at 450 nm
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Plaque

    Pre-coated

    Protocole

    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
    3. Add 100 μL prepared HRP-anti positive control IgA Antibody and/or 100 μL of prepared HRP-anti Human IgA Antibody to each well. Incubate 1 hour at room temperature.
    4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
    5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

    Restrictions

    For Research Use only
  • Stock

    -80 °C

    Stockage commentaire

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
  • Antigène Voir toutes IgA Kits ELISA

    IgA

    Classe de substances

    Antibody

    Sujet

    Gene Symbols: IGA
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