IgG Kit ELISA

N° du produit ABIN8069248

Le kit ELISA IgG (ABIN8069248) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain IgG.

Aperçu rapide pour IgG Kit ELISA (ABIN8069248)

Antigène: IgG

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit IgG Kit ELISA

Fonction: COVID-19 Beta Spike Variant Human IgG ELISA Kit 3 is an antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the spike protein mutations (K417N, E484K, N501Y) associated with the Beta variant of SARS-CoV-2. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytical Method: Semi-Quantitative

Ingrédients:

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

,

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Gene Symbols: IGG