IgG Kit ELISA

N° du produit ABIN8069265

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain IgG.

Aperçu rapide pour IgG Kit ELISA (ABIN8069265)

Antigène: IgG

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit IgG Kit ELISA

Fonction: COVID-19 IHU Spike Variant Human IgG ELISA Kit is an antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the spike protein mutations (R346S, N394S, Y449N, E484K, F490R, N501Y) associated with the IHU variant of SARS-CoV-2. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytical Method: Semi-Quantitative

Ingrédients:

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

,

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Gene Symbols: IGG