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IgM Kit ELISA

Name: IgM Kit ELISA
SKU: ABIN8069632
Availability: InStock

Le kit ELISA IgM (ABIN8069632) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain IgM.

N° du produit ABIN8069632

533,68 €

Frais d'envoi 40,00 €, glace carbonique 20,00 € et TVA exclus

96 tests

Destination: France

Envoi sous 6 à 9 jours ouvrables

Aperçu rapide pour IgM Kit ELISA (ABIN8069632)

Antigène

Voir toutes IgM Kits ELISA

IgM

Reactivité

Humain

Méthode de détection

Colorimetric

Type de méthode

Indirect ELISA

Application

ELISA

Type d'échantillon

Serum

Discover our top product IgM Kit ELISA

Fonction

COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytical Method

Semi-Quantitative

Ingrédients
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Negative Control
- HRP-anti positive control IgM Antibody
- HRP-anti Human IgM Antibody
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
,
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Negative Control
- HRP-anti positive control IgM Antibody
- HRP-anti Human IgM Antibody
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
Matériel non inclus
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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Indications d'application

Optimal working dilution should be determined by the investigator.

Plaque

Pre-coated

Protocole
1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP-anti positive control IgM Antibody and/or 100 μL of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
Restrictions

For Research Use only
Stock

-80 °C

Stockage commentaire

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.
Antigène Voir toutes IgM Kits ELISA

IgM

Classe de substances

Antibody

Sujet

Gene Symbols: IGM