IgM Kit ELISA

N° du produit ABIN8069639

Kit ELISA IgM Humain, Colorimetric test pour la quantification de Humain IgM.

Aperçu rapide pour IgM Kit ELISA (ABIN8069639)

Antigène: IgM

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit IgM Kit ELISA

Fonction: COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytical Method: Semi-Quantitative

Ingrédients:

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Negative Control
• HRP-anti positive control IgM Antibody
• HRP-anti Human IgM Antibody
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent

,

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Negative Control
• HRP-anti positive control IgM Antibody
• HRP-anti Human IgM Antibody
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP-anti positive control IgM Antibody and/or 100 μL of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.

Détail du antigène

Antigène: IgM

Classe de substances: Antibody

Sujet: Gene Symbols: IGM