Lipopolysaccharides (LPS) Kit ELISA

N° du produit ABIN8069971

Le kit ELISA Lipopolysaccharides (LPS) (ABIN8069971) E. coli est un kit ELISA Colorimetric conçu pour quantifier E. coli Lipopolysaccharides (LPS).

Aperçu rapide pour Lipopolysaccharides (LPS) Kit ELISA (ABIN8069971)

Antigène: Lipopolysaccharides (LPS)

Reactivité: E. coli

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.01 ng/mL - 12 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate

Détail du produit Lipopolysaccharides (LPS) Kit ELISA

Seuil minimal de détection: 0.01 ng/mL

Fonction: Escherichia coli Lipopolysaccharide ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of process-related LPS, the common endotoxin impurities, derived from production processes when E. coli strains are utilized.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects E. coli LPS. The possibility of this antibody pair to detect LPS of other gram-negative bacteria has not been tested.

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: Lipopolysaccharides (LPS)

Autre désignation: LPS

Classe de substances: Chemical