MANF Kit ELISA

N° du produit ABIN8070036

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain MANF.

Aperçu rapide pour MANF Kit ELISA (ABIN8070036)

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.013 ng/mL - 5 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit MANF Kit ELISA

Seuil minimal de détection: 0.013 ng/mL

Fonction: Human MANF ELISA kit for Serum and Plasma samples.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human MANF. Other species not determined.

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Information d'application

Indications d'application: Suggested Plasma Dilution: 2-10 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: MANF

Sujet:

Gene Symbols: MANF

Protein Name (Synonyms): Mesencephalic astrocyte-derived neurotrophic factor (Arginine-rich protein) (Protein ARMET)

ID gène: 7873

UniProt: P55145