Nuclease Kit ELISA

N° du produit ABIN8070291

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Serratia marcescens Nuclease.

Aperçu rapide pour Nuclease Kit ELISA (ABIN8070291)

Antigène: Nuclease

Reactivité: Serratia marcescens

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 19.92 pg/mL - 9600 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant

Détail du produit Nuclease Kit ELISA

Seuil minimal de détection: 19.92 pg/mL

Fonction: Nuclease ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of nucleases, including SuperNuclease® and Benzonase®, in cell culture supernatants and purified biological products while nuclease is supplied to remove host cell DNA during manufacturing.

Analytical Method: Quantitative

Specificité: This ELISA kit has been validated with SuperNuclease®, Benzonase® and RayBio® Universal Nuclease, and should react with other Serratia marcescens nuclease products but not tested. Both non-denatured and denatured nuclease can be detected by this kit.

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: Nuclease

Sujet:

Gene Symbols: nucA nuc

Protein Name (Synonyms): Nuclease (EC 3.1.30.2) (Endonuclease) [Cleaved into: Nuclease isoform Sm2, Nuclease isoform Sm3, Nuclease isoform Sm1

UniProt: P13717