PD-1 Kit ELISA

N° du produit ABIN8070494

Kit ELISA PD-1 Humain, Colorimetric test pour la quantification de Humain PD-1.

Aperçu rapide pour PD-1 Kit ELISA (ABIN8070494)

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Épitope: phosphorylated

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Lysate

Détail du produit PD-1 Kit ELISA

Fonction: Phosphotyrosine PD-1 ELISA Kit is a convenient and sensitive assay to detect Phosphotyrosine PD-1 in human cell lysate samples.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human Phospho PD-1.

Ingrédients:

• Pre-coated 96-well microplate
• Positive Control
• Anti-phospho Detection Antibody
• HRP-Streptavidin
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL of positive control and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Streptavidin to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Autre désignation: PD1

Sujet:

Gene Symbols: PDCD1, PD1

Protein Name (Synonyms): Programmed cell death protein 1 (Protein PD-1, hPD-1)

ID gène: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints