tau Kit ELISA

N° du produit ABIN8070982

Le kit ELISA tau (ABIN8070982) Humain, Souris et Rat est un kit ELISA Colorimetric conçu pour quantifier Humain, Souris et Rat tau.

Aperçu rapide pour tau Kit ELISA (ABIN8070982)

Antigène: tau (tau Protein)

Épitope: pThr217

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.37 ng/mL - 90 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Lysate

Détail du produit tau Kit ELISA

Seuil minimal de détection: 0.37 ng/mL

Fonction: Phospho-TAU (Thr217) Quantitative ELISA Kit is a convenient and sensitive assay to detect phosphorylated Tau at Threonine-217 (p-Tau217) in human cell lysate samples.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human, mouse and rat Phospho TAU (Thr217).

Sensibilité: 0.09 ng/mL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Détail du antigène

Antigène: tau (tau Protein)

Autre désignation: Tau

Sujet:

Gene Symbols: MAPT MAPTL MTBT1 TAU

Protein Name (Synonyms): Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

ID gène: 4137

UniProt: P10636