Triiodothyronine T3 Kit ELISA

N° du produit ABIN8071151

Kit ELISA Triiodothyronine T3 Humain, Rat, Chien, Souris, Porc, Mouton, Cheval et Cat, Colorimetric test pour la quantification de Humain, Rat, Chien, Souris, Porc, Mouton, Cheval et Cat Triiodothyronine T3.

Aperçu rapide pour Triiodothyronine T3 Kit ELISA (ABIN8071151)

Antigène: Triiodothyronine T3 (T3)

Reactivité: Humain, Rat, Chien, Souris, Porc, Mouton, Cheval, Cat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.0002 ng/dL - 200 ng/dL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Saliva, Serum, Urine

Détail du produit Triiodothyronine T3 Kit ELISA

Seuil minimal de détection: 0.0002 ng/dL

Fonction: This kit is a competitive immunoassay designed to quantify free T3 in human, mouse, rat, porcine, feline, canine, equine, and sheep serum or plasma, as well as urine samples, within 2.5 hours.

Analytical Method: Quantitative

Specificité: This EIA kit detects Triiodothyronine / T3

Sensibilité: 0.002 ng/dL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated Secondary Antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary Antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Triiodothyronine T3 (T3)

Autre désignation: Triiodothyronine

Classe de substances: Amino Acid

Sujet: Optional[Gene Symbols]: Free T3 (FT3) is the unbound, biologically active form of triiodothyronine (T3), the thyroid hormone that directly regulates metabolism at the cellular level. Measuring FT3 indicates how much hormone is actually available to enter cells and exert physiological effects. Only about 0.3 % of total T3 is free, the remainder is bound to proteins and biologically inactive. Therefore, FT3 measurement can reflect thyroid hormone activity, peripheral T4-to-T3 conversion efficiency, and tissue-level thyroid hormone effects.

The Free T3 Enzyme Immunoassay (EIA) Kit is a competitive immunoassay that quantifies T3 in serum or plasma from human, mouse, rat, porcine, feline, canine, equine, and sheep samples, as well as urine samples within 2.5 hours. Absorbance is measured at 450 nm, and concentrations are interpolated from a ready-to-use T3 standard curve. The assay demonstrates a sensitivity of 0.002 ng/dL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.