Triiodothyronine T3 Kit ELISA

N° du produit ABIN8071155

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain, Rat, Chien, Porc, Souris, Mouton, Cheval et Cat Triiodothyronine T3.

Aperçu rapide pour Triiodothyronine T3 Kit ELISA (ABIN8071155)

Antigène: Triiodothyronine T3 (T3)

Épitope: total

Reactivité: Humain, Rat, Chien, Porc, Souris, Mouton, Cheval, Cat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.02 ng/dL - 20000 ng/dL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Saliva, Serum, Urine

Détail du produit Triiodothyronine T3 Kit ELISA

Seuil minimal de détection: 0.02 ng/dL

Fonction: This kit is a competitive immunoassay designed to quantify total T3 in human, mouse, rat, porcine, feline, canine, equine, and sheep serum or plasma, as well as urine samples, within 3 hours.

Analytical Method: Quantitative

Specificité: This EIA kit detects Triiodothyronine / T3

Sensibilité: 0.167 ng/dL

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Treatment Buffer
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated Secondary Antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary Antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Triiodothyronine T3 (T3)

Autre désignation: Triiodothyronine

Classe de substances: Amino Acid

Sujet: Optional[Gene Symbols]: Total triiodothyronine (TT3) represents the entire circulating pool of T3, encompassing both the protein-bound (~99.7 % ) and the biologically active free fraction (~0.3 %). Approximately 20 % of T3 is secreted directly by the thyroid gland, whereas 80 % arises from peripheral conversion of T4 in liver, muscle, kidney, and brain. Clinically, elevated TT3 may indicate hyperthyroidism, T3 toxicosis (elevated T3 with normal T4), or excessive thyroid hormone replacement. Conversely, reduced TT3 is observed in advanced hypothyroidism, severe systemic illness, starvation, chronic stress, and non-thyroidal illness syndrome (euthyroid sick syndrome).

The Total T3 Enzyme Immunoassay (EIA) Kit is a competitive immunoassay designed to quantify T3 in human, mouse, rat, porcine, feline, canine, equine, and sheep serum or plasma, as well as urine samples, within 3 hours. Absorbance is measured at 450 nm, and concentrations are interpolated from a ready-to-use T3 standard curve. The assay demonstrates a sensitivity of 0.167 ng/dL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.