TRIM21 Kit ELISA

N° du produit ABIN8071161

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain TRIM21.

Aperçu rapide pour TRIM21 Kit ELISA (ABIN8071161)

Antigène: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Gamme de detection: 1.37 units/mL - 1000 units/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit TRIM21 Kit ELISA

Seuil minimal de détection: 1.37 units/mL

Fonction: The Sjogren Syndrome Antigen (SSA) Human IgA ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against SSA antigen(s) in human serum or plasma.

Analytical Method: Quantitative

Ingrédients:

Size / Description
• SSA Coated 96-Well Microplate
• 96 wells coated with SSA
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgA
• HRP-Streptavidin
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent
• Sample Diluent

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Suggested Plasma Dilution: 1,000-fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgA Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The unopened kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Autre désignation: TRIM21

Sujet:

Gene Symbols: TRIM21 RNF81 RO52 SSA1

Background: The Sjogren Syndrome Antigen (SSA) Human IgA ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with SSA protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-SSA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): E3 ubiquitin-protein ligase TRIM21 (EC 2.3.2.27) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21)

ID gène: 6737

UniProt: Q5XPV5, Q96RF8