ATG7 Kit ELISA
Aperçu rapide pour ATG7 Kit ELISA (ABIN8075032)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.156 ng/mL
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Fonction
- Mouse Autophagy Related Protein 7 (ATG7) ELISA Kit for detecting ATG7 in tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensibilité
- 0.057 ng/mL
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Ingrédients
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Matériel non inclus
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 h
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Plaque
- Pre-coated
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Précision du teste
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- 4°C for one month, Long term: see manual.
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Date de péremption
- 12 months
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- ATG7 (ATG7 Autophagy Related 7 (ATG7))
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Autre désignation
- Autophagy Related Protein 7
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Sujet
- Gene Aliases: APG7L, GSA7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Ubiquitin-activating enzyme E1-like protein
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ID gène
- 74244
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UniProt
- Q9D906
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Pathways
- Response to Water Deprivation, Autophagy
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