Lipopolysaccharides (LPS) Kit ELISA

N° du produit ABIN8090868

Le kit ELISA Lipopolysaccharides (LPS) (ABIN8090868) Différentes espèces est un kit ELISA Colorimetric conçu pour quantifier Différentes espèces Lipopolysaccharides (LPS).

Aperçu rapide pour Lipopolysaccharides (LPS) Kit ELISA (ABIN8090868)

Antigène: Lipopolysaccharides (LPS)

Reactivité: Différentes espèces

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.313 μg/mL - 20 μg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit Lipopolysaccharides (LPS) Kit ELISA

Seuil minimal de détection: 0.313 μg/mL

Fonction: LPS (Lipopolysaccharides) ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with LPS , no obvious cross reaction with other analogues.

Sensibilité: 0.188 μg/mL

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Competitive ELISA, Coated with Antibody

Durée du test: 2 h

Plaque: Pre-coated

Protocole: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/biotin-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: Lipopolysaccharides (LPS)

Autre désignation: LPS

Classe de substances: Chemical

Sujet: LPS, Lipopolysaccharides