CXCL9 Kit ELISA

N° du produit ABIN8126235

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain CXCL9.

Aperçu rapide pour CXCL9 Kit ELISA (ABIN8126235)

Antigène: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit CXCL9 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 94 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human CXCL9 concentrations in serum, plasma. Detection Range: 156 - 10,000 pg/mL Sensitivity: <94 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human CXCL9 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human CXCL9 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CXCL9 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human CXCL9 can be calculated using the standard curve.  

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Autre désignation: CXCL9/MIG (C-X-C motif chemokine 9)