Osteopontin Kit ELISA

N° du produit ABIN8126238

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Osteopontin.

Aperçu rapide pour Osteopontin Kit ELISA (ABIN8126238)

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Osteopontin Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate.

Analytical Method: Quantitative

Sensibilité: 93.75 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human OPN concentrations in serum, plasma.  Detection Range: 0.156 - 10 ng/mL Sensitivity: < 93.75 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate. Standards and samples are pipetted into the wells and OPN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human OPN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of OPN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Autre désignation: OPN (Osteopontin)

Pathways: Regulation of Cell Size