IL-10 Kit ELISA

N° du produit ABIN8126244

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris IL-10.

Aperçu rapide pour IL-10 Kit ELISA (ABIN8126244)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-10 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate.

Analytical Method: Quantitative

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma.  Detection Range: 15.6 - 1000 pg/mL Sensitivity: <9.4pg / ml

Information d'application

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL-10 (Interleukin 10)

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints