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Cathepsin B Kit ELISA

CTSB Reactivité: Souris AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN921085
  • Antigène Voir toutes Cathepsin B (CTSB) Kits ELISA
    Cathepsin B (CTSB)
    Épitope
    AA 18-339
    Reactivité
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cathepsin B
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: H18-F339
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <5pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: H18-F339
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO, H18-F339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin B amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Cathepsin B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Cathepsin B standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 1154, Standard deviation: 61.2, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 2875, Standard deviation: 161.0, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 5823, Standard deviation: 355.2, CV(%): 6.1,
    • Sample 1: n=24, Mean(pg/ml): 1298, Standard deviation: 102.5, CV(%): 7.9
    • Sample 2: n=24, Mean(pg/ml): 2794, Standard deviation: 231.9, CV(%): 8.3
    • Sample 3: n=24, Mean(pg/ml): 5940, Standard deviation: 528.7, CV(%): 8.9
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Cathepsin B (CTSB) Kits ELISA
    Cathepsin B (CTSB)
    Autre désignation
    CTSB (CTSB Produits)
    Synonymes
    APPS Kit ELISA, CPSB Kit ELISA, CB Kit ELISA, apps Kit ELISA, cpsb Kit ELISA, LOC692390 Kit ELISA, CTSB Kit ELISA, cathepsin B Kit ELISA, cathepsin B S homeolog Kit ELISA, CTSB Kit ELISA, Ctsb Kit ELISA, ctsb.S Kit ELISA, LOC692390 Kit ELISA
    Sujet

    Protein Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

    Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

    Synonyms: Cathepsin B,3.4.22.1,Cathepsin B1,Cathepsin B light chain,Cathepsin B heavy chain,Ctsb,

    Full Gene Name: Cathepsin B

    Cellular Localisation: Lysosome. Melanosome . Secreted, extracellular space.
    ID gène
    13030
    UniProt
    P10605
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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