RBP4 Kit ELISA

N° du produit ABIN921108

Ce kit ELISA Souris RBP4 est un kit ELISA Colorimetric conçu pour quantifier Souris RBP4.

Aperçu rapide pour RBP4 Kit ELISA (ABIN921108)

Antigène: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Épitope: AA 19-201

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Détail du produit RBP4 Kit ELISA

Seuil minimal de détection: 312 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse RBP4

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: E19-L201

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: E19-L201

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.

Plaque: Pre-coated

Protocole: mouse RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RBP4 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse RBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin , EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse RBP4 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.34, Standard deviation: 0.06, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 4.6, Standard deviation: 0.348, CV(%): 5.7
• Sample 3: n=16, Mean(ng/ml): 10.12, Standard deviation: 0.617, CV(%): 6.1,
• Sample 1: n=24, Mean(ng/ml): 1.65, Standard deviation: 0.102, CV(%): 6.2
• Sample 2: n=24, Mean(ng/ml): 4.34, Standard deviation: 0.304, CV(%): 7
• Sample 3: n=24, Mean(ng/ml): 9.86, Standard deviation: 0.76, CV(%): 7.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Autre désignation: RBP4

Sujet:

Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37-K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Synonyms: Retinol-binding protein 4 ,Rbp4 ,

Full Gene Name: retinol binding protein 4, plasma

ID gène: 19662

UniProt: H7BWY6

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response