IL23A Kit ELISA

N° du produit ABIN921111

Le kit ELISA IL23A (ABIN921111) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris IL23A. Ce kit ELISA a été cité dans 1 publication.

Aperçu rapide pour IL23A Kit ELISA (ABIN921111)

Antigène: IL23A (Interleukin 23, alpha subunit p19 (IL23A))

Épitope: AA 20-196, AA 23-335

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit IL23A Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Mouse IL-23 ELISA Kit to quantitate Mouse Il23a in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: (M23-S335) + (L20-A196)

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.4%, 5%, 4.4%
Inter-Assay Precision (CV%): 6.2%, 6.9%, 5.1%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour IL23A Kit ELISA (ABIN921111)

Zhang, Ding, Zhao, Yang, Zhang, Zhang, Cui: "Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats." dans: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1821-8, (2009) (PubMed).

Détail du antigène

Antigène: IL23A (Interleukin 23, alpha subunit p19 (IL23A))

Autre désignation: IL23A

Sujet:

Background: Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).

Gene Full Name: interleukin 23, alpha subunit p19

ID gène: 83430

UniProt: Q9EQ14

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation