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HAVCR1 Kit ELISA

HAVCR1 Reactivité: Rat AA 18-238 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
N° du produit ABIN921116
  • Antigène Voir toutes HAVCR1 Kits ELISA
    HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
    Épitope
    AA 18-238
    Reactivité
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat KIM1
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: S18-V238
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <2pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: S18-V238
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the immunoglobulin superfamily. TIM family.

    Tissue Specificity: Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. .

    Plaque
    Pre-coated
    Protocole
    rat KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, S18-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat KIM1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat KIM1 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 276, Standard deviation: 11.6, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 662, Standard deviation: 35.1, CV(%): 5.3
    • Sample 3: n=16, Mean(pg/ml): 1186, Standard deviation: 41.51, CV(%): 3.5,
    • Sample 1: n=24, Mean(pg/ml): 359, Standard deviation: 22.26, CV(%): 6.2
    • Sample 2: n=24, Mean(pg/ml): 710, Standard deviation: 48.28, CV(%): 6.8
    • Sample 3: n=24, Mean(pg/ml): 1332, Standard deviation: 75.9, CV(%): 5.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Qiu, Sun, Zhang, Li, Wang: "Involvement of the NF-κB signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model." dans: Biomedical reports, Vol. 4, Issue 5, pp. 628-634, (2016) (PubMed).

    Li, Ma, Zhao, Yuan, Li, Wang: "Glycyrrhetinic acid might increase the nephrotoxicity of bakuchiol by inhibiting cytochrome P450 isoenzymes." dans: PeerJ, Vol. 4, pp. e2723, (2016) (PubMed).

  • Antigène Voir toutes HAVCR1 Kits ELISA
    HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
    Autre désignation
    HAVCR1 (HAVCR1 Produits)
    Synonymes
    HAVCR Kit ELISA, HAVCR-1 Kit ELISA, KIM-1 Kit ELISA, KIM1 Kit ELISA, TIM Kit ELISA, TIM-1 Kit ELISA, TIM1 Kit ELISA, TIMD-1 Kit ELISA, TIMD1 Kit ELISA, Kim1 Kit ELISA, HAVCR1 Kit ELISA, LOC100226241 Kit ELISA, AI503787 Kit ELISA, Tim1 Kit ELISA, Timd1 Kit ELISA, hepatitis A virus cellular receptor 1 Kit ELISA, hepatitis A virus cellular receptor 1 homolog Kit ELISA, HAVCR1 Kit ELISA, Havcr1 Kit ELISA, LOC100226241 Kit ELISA
    Classe de substances
    Virus
    Sujet

    Protein Function: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity). .

    Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

    Synonyms: Hepatitis A virus cellular receptor 1 homolog,HAVcr-1,Kidney injury molecule 1,KIM-1,T cell immunoglobulin and mucin domain-containing protein 1,TIMD-1,Havcr1,Kim1,

    Full Gene Name: Hepatitis A virus cellular receptor 1 homolog

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    286934
    UniProt
    O54947
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