HBsAg PreS1 Kit ELISA

N° du produit

Kit ELISA Cobaye HBsAg PreS1, test Colorimetric pour la quantification de Cobaye HBsAg PreS1.

Aperçu rapide pour HBsAg PreS1 Kit ELISA ()

Antigène: HBsAg PreS1 (HBV PreS1) (Hepatitis B Surface Antigen PreS1 (HBV PreS1))

Reactivité: Cobaye

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Détail du produit HBsAg PreS1 Kit ELISA

Highlights:

• Kit de dosage de la taurine fiable pour la mesure de la taurine libre dans les échantillons biologiques.
• Les concentrations de taurine dans les échantillons sont déterminées par comparaison avec un étalon de taurine connu.
• Rapide et facile à utiliser.

Highlights:

• Anticorps polyclonal non conjugué de la GFP pour une détection fiable de la GFP et de ses variantes.
• Validé pour la microscopie à fluorescence, ELISA, Western Blotting.
• Anticorps GFP de haute qualité, cité dans plus de 177 références PubMed.
• Disponible en quantités de 10 µl et 100 µl.

Highlights:

• Billes magnétiques d'agarose Concanavalin A pour la chromatographie d'affinité à l'aide d'un séparateur magnétique.
• Purification facile des virus, des particules de type virus (VLP) et des cellules cultivées via la liaison réversible des glycoprotéines, des glycolipides ou des polysaccharides avec le mannose ou le glucose terminal à la concanavaline A.

Highlights:

• Kit ELISA pour la détection quantitative de l'IL-1 beta chez la souris.
• Produit fiable avec des composants validés.
• Kit ELISA cité dans plus de 40 références PubMed.

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• L'anticorps anti-CRBN de lapin détecte de manière fiable le CRBN chez l'homme.
• Anticorps polyclonal non conjugué pour ELISA, WB, IHC.
• Détection hautement spécifique de CRBN. Voir les images de validation.

Highlights:

• L'anticorps de lapin contre la bêta-caténine détecte de manière fiable les cibles de la bêta-caténine dans la signalisation Wnt en utilisant CUT&RUN, ICC, IF, WB, ELISA.
• Convient parfaitement à l'imagerie des jonctions d'adhérence en IHC/IF.
• Détection hautement spécifique de la bêta-caténine en WB.

Highlights:

• Fréquemment cité dans des publications scientifiques avec plus de 300 citations
• Validé en interne pour des applications pertinentes
• Rapport de validation détaillé de l'IF fourni par l'un de vos pairs
• Conçu pour détecter le RFP et ses variantes

Highlights:

• Détection d'impuretés dans la fabrication de médicaments biologiques.
• Le kit AccuSignal™ Nuclease ELISA est conçu pour une quantification sensible et fiable des nucléases dans les produits thérapeutiques, y compris DENARASE®, Benzonase® et Turbonuclease.
• Il offre un large spectre de quantification, tout en conservant une linéarité de dilution exceptionnelle, ce qui permet d'avoir confiance dans la précision des résultats sur un large éventail de concentrations de nucléases.
• Les résultats sont cohérents et reproductibles, caractérisés par une variabilité minimale intra- et inter-essais.
• La spécificité de l'anticorps permet une application sur divers matériaux, s'adaptant à divers produits de nucléases provenant de plusieurs fournisseurs.
• Composants : Plaque à 96 puits, scelleur de plaque, nucléase standard, anticorps de détection biotinylé, Streptavidine-HRO (100X), tampons.

Highlights:

• Anticorps FcRn de haute qualité (Clone DVN24) pour la détection de FcRn.
• Hautement spécifique du FcRn humain, bloque la liaison de l'IgG.
• PBS-only, preservative free.
• 2 références PubMed disponibles pour cet anticorps FcRn.

Highlights:

• Anticorps FcRn de haute qualité (Clone ADM31) pour la détection de FcRn.
• Hautement spécifique pour le FcRn humain, bloque la liaison de l'albumine sérique humaine.
• PBS-only, preservative free
• 15 références PubMed disponibles pour cet anticorps FcRn.

Highlights:

• Anticorps anti-GFP à domaine unique (sdAb) de haute affinité lié de manière covalente à des billes magnétiques d'agarose réticulées à 4 % d'un diamètre de 50 à 150 µm.
• L'anticorps sdAb Alpaca clone 1H1 est spécifique de la GFP et de nombreux dérivés de la GFP. Capacité >3 µg de GFP / 1 µL de billes emballées.
• Compatible avec les tampons physiologiques, les tampons de lyse et de lavage courants et les tampons à haute stringence.

Highlights:

• Anticorps anti-GFP à domaine unique (sdAb) de haute affinité lié de manière covalente à des billes d'agarose réticulées à 4 % d'un diamètre de 50 à 150 µm.
• L'anticorps sdAb Alpaca clone 1H1 est spécifique de la GFP et de nombreux dérivés de la GFP. Capacité >3 µg de GFP / 1 µL de billes emballées.
• Compatible avec les tampons physiologiques, les tampons de lyse et de lavage courants et les tampons à haute stringence.

Highlights:

• TrueBlot®: Anti-Rabbit IgG HRP, Mouse Monoclonal eB182
• Fabriquée par Rockland Immunochemicals, Inc.
• Rockland Product ID: 18-8816-33

Highlights:

• TrueBlot®: Anti-Rabbit IgG HRP, Mouse Monoclonal eB182
• Fabriquée par Rockland Immunochemicals, Inc.
• Rockland Product ID: 18-8816-31

Highlights:

• Anticorps monoclonal anti-ATM protéine kinase pS1981 (MOUSE)
• Fabriqué par Rockland Immunochemicals, Inc.
• ID du produit Rockland : 200-301-400

Highlights:

• Anticorps FcRn de haute qualité (Clone ADM31) pour la détection de FcRn.
• Hautement spécifique pour le FcRn humain, bloque la liaison de l'albumine sérique humaine.
• Contient du glycérol pour une meilleure conservation.

Highlights:

• Anticorps FcRn de haute qualité (Clone DVN24) pour la détection de FcRn.
• Hautement spécifique du FcRn humain, bloque la liaison de l'IgG.
• Contient du glycérol pour une meilleure conservation.

Fonction: This ELISA kit is a solid phase ELISA designed for quantitative determination of HBsAg preS1.

Ingrédients:

• Microtiter plate (96 wells stripwell) - 1
• Enzyme conjugate - 1 vial
• Standard A - 1 vial
• Standard B - 1 vial
• Standard C - 1 vial
• Standard D - 1 vial
• Standard E - 1 vial
• Standard F - 1 vial
• Substrate A - 1 vial
• Substrate B - 1 vial
• Stop solution - 1 vial
• Wash solution - 1 vial
• Balance solution - 1 vial
• Instruction manual - 1

Matériel non inclus:

• Precision pipettors and disposable tips to deliver 10-1000µL. A multi-channel pipette is desirable for large assays.
• 100mL and 1L graduated cylinders.
• Distilled or deionized water
• Tubes to prepare sample dilutions.
• Absorbent paper.
• Microplate reader capable of measuring absorbance at 450nm.
• Centrifuge capable of 3000 × g.
• Microplate washer or washing bottle.
• Incubator (37°C).
• Data analysis and graphing software.

Informations sur le produit:

À quoi peut servir l'anticorps IL-1 bêta ABIN6574166 ? Le kit ELISA IL-1 beta peut être utilisé pour analyser l'expression de la protéine IL1 beta dans le surnageant de culture cellulaire, les lysats cellulaires, le plasma, le sérum, l'homogénat de tissu. Le volume d'échantillon requis est de 100 µl. Le kit ELISA est validé pour la souris et a une gamme de détection de 15.6 pg/mL - 1000 pg/mL pour l'Interleukine beta 1 avec une sensibilité de 5.7 pg/mL. Il ne présente pas d'interférence significative ni de réactivité croisée entre l'IL-1 bêta et les analogues. Utilisez le kit ELISA IL-1 beta de souris pour la détection quantitative de l'IL-1 beta par ELISA sandwich en 3 heures. Il présente une sensibilité élevée et une excellente spécificité.

Quelles sont les données de validation disponibles pour cet anticorps IL-1 bêta ? Le produit est cité dans 41 publications PubMed. Les images de validation des composants du kit ELISA anticorps de détection, anticorps de capture et standard protéique se trouvent ci-dessus. Pour une utilisation de recherche dans le secteur académique ou industriel uniquement.

Quelle est la fonction de l'IL-1 beta / Interleukine 1 beta ? L'IL-1 bêta (interleukine 1 bêta) appartient à la famille des interleukines et est une puissante cytokine pro-inflammatoire. Découverte à l'origine comme le plus important pyrogène endogène, l'IL-1 bêta induit la synthèse des prostaglandines, l'afflux et l'activation des neutrophiles, l'activation des lymphocytes T, la production de cytokines, l'activation des lymphocytes B, la production d'anticorps, ainsi que la prolifération des fibroblastes et la production de collagène. L'IL-1 bêta joue un rôle dans l'angiogenèse en induisant la production de VEGF de manière synergique avec le TNF et l'IL6.

Information d'application

Indications d'application:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of samples used in the whole test. Please reserve sufficient amounts of samples in advance.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Commentaires:

• It is recommended that all standards, controls and samples be run in duplicate. Standards and samples must be assayed at the same time.
• The coefficient of determination of the standard curve should be higher or equal 0.95 and the highest O.D. should be more than 1.0.
• Cover or cap all kit components and store at 2-8°C when not in use.
• Microtiter plates should be allowed to come to room temperature before opening the foil bags. Once the desired number of strips has been removed, immediately reseal the bag with desiccants and store at 2-8°C to maintain plate integrity.
• Samples should be collected in pyrogen/endotoxin-free tubes.
• Samples should be frozen if not analyzed shortly after collection. Avoid multiple freeze-thaw cycles of frozen samples. Thaw completely and mix well prior to analysis.
• When possible, avoid use of badly hemolyzed or lipemic serum. If large amounts of particulate matter are present, centrifuge or filter prior to analysis.
• When pipetting reagents, maintain a consistent order of addition from well-to-well. This ensures equal incubation times for all wells.
• Do not mix or interchange different reagent lots from various kit lots.
• Do not use reagents after the kit expiration date.
• Read absorbance immediately after adding the stop solution.
• Incomplete washing will adversely affect the test outcome. All washing must be performed with Wash Solution provided. All residual wash liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
• Because TMB is light sensitive, avoid prolonged exposure to light. Also avoid contact between TMB and metal, otherwise color may develop.

Information on standard material:
Different kits have different standards. For kits detecting proteisn or peptidse, the standards are recombinant proteins or synthetic peptides. For kits detecting small chemical compounds, the standards are synthetic chemical compounds. There are no standards extracted from natural resources. All of our reombinant proteins are expressed in E.coli. The standard are dissolved in PBS with 0.1 % proclin 300 and some other preservatives.

Information on reagents:
The STOP solution is 1M sulphuric acid. The wash buffer is 0.05 % Tween 20 in PBS, pH 7.4. The ELISA kit dose not contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME). Part of the reagents contain BSA.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Durée du test: 1.5 h

Plaque: Strips (12 x 8)

Préparation des réactifs:

• Samples - Please predict the concentration before assaying. If concentrations are unknown or not within the detection range, a preliminary experiment is recommended to determine the optimal dilution. PBS (pH 7.0-7.2) or 0.9% physiological saline can be used as dilution buffer.
• Wash solution - Dilute 10mL of wash solution concentrate (100×) with 990mL of deionized or distilled water to prepare 1000mL of wash solution (1×). If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have dissolved. The 1× wash solution is stable for 2 weeks at 2-8°C.

Note:

• Bring all kit components and samples to room temperature (20-25°C) before use.
• Do not dilute other ready-to-use components.

Prélèvement de l'échantillon:

• Serum: Use a serum separator tube and allow samples to clot for 2 hours at room temperature or overnight at 2-8°C. Centrifuge at approximately 1000 × g (or 3000rpm) for 15 minutes. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C.
• Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 100 × g (or 3000rpm) at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C.
• Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, thoroughly rinse tissues in ice-cold PBS (0.02mol/L, pH 7.0-7.2) to remove excess blood and weigh before homogenization. Mince the tissues into small pieces and homogenize them in a certain amount of PBS with a glass homogenizer on ice. Subject the resulting suspension to ultrasonication or to two freeze-thaw cycles to further break down cell membranes. After that, centrifuge for 15 minutes at 1500 × g (or 5000rpm). Remove the supernate and assay immediately or aliquot and store samples at -20°C or -80°C.
• Cell lysates: Cells should be lysed according to the following directions.
  • 1. Adherent cells should be detached with trypsin and then collected by centrifugation. Suspension cells can be collected by centrifugation directly.
  • 2. Wash three times in PBS.
  • 3. Resuspend cells in PBS and subject to ultrasonication 3 times. Alternatively, freeze cells at -20°C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle 3 times.
  • 4. Centrifuge at 1000 × g (or 3000rpm) for 15 minutes at 2-8°C to remove cellular debris.
  • 5. Assay immediately or store samples at -20°C or -80°C.
• Cell culture supernatants and other body fluids: Centrifuge cell culture media at 1000 × g (or 3000rpm) for 15 minutes to remove debris. Assay immediately or store samples at -20°C or -80°C.

Note:

• Samples should be aliquoted and must be stored at -20°C (lower or equal 3 months) or -80°C (lower or equal 6 months) to avoid loss of bioactivity and contamination. If samples are to be run within 24 hours, they may be stored at 2-8°C. Avoid repeated freeze-thaw cycles.
• Prior to assay, the frozen sample should be brought to room temperature slowly and mixed gently.
• Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
• Samples containing a visible precipitate must be clarified prior to use in the assay. Care should be taken to minimize hemolysis. Do not use grossly hemolyzed or lipemic specimens.
• Do not use heat-treated specimens.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation:

• This kit contains a small amount of 3, 3’, 5, 5’-Tetramethylbenzidine (TMB) in Substrate B. TMB is non-carcinogenic but it is hazardous in case of skin contact, eye contact, ingestion and inhalation. In case of contact, rinse affected area with plenty of water.
• The stop solution provided with this kit is an acid solution. Wear protective gloves, clothing, and face protection.
• Care should be taken when handling the standard because of the known and unknown effects of it.
• Care should also be taken to avoid contact of skin or eyes with other kit reagents or specimens. In the case of contact, wash immediately with water.
• Do not pipette by mouth.
• Avoid generation of aerosols.
• Waste must be disposed of in accordance with federal, state and local environmental control regulations.
• All blood components and biological materials should be handled as potentially hazardous. Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour a 121.5°C.

Conseil sur la manipulation:

• The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient amount of samples in advance.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Owing to the possibility of mismatching between antigen from other resource and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
• Influenced by the factors including cell viability, cell number and also sampling time, samples from cell culture supernatant may not be detected by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
• It is recommended that all standards, controls and samples be run in duplicate. Standards and samples must be assayed at the same time.
• The coefficient of determination of the standard curve should be higher or equal 0.95 and the highest O.D. should be more than 1.0.
• Cover or cap all kit components and store at 2-8° C when not in use.
• Microtiter plates should be allowed to come to room temperature before opening the foil bags. Once the desired number of strips has been removed, immediately reseal the bag with desiccants and store at 2-8°C to maintain plate integrity.
• Samples should be collected in pyrogen/endotoxin-free tubes.
• Samples should be frozen if not analyzed shortly after collection. Avoid multiple freeze-thaw cycles of frozen samples. Thaw completely and mix well prior to analysis.
• When possible, avoid use of badly hemolyzed or lipemic serum. If large amounts of particulate matter are present, centrifuge or filter prior to analysis.
• When pipetting reagents, maintain a consistent order of addition from well-to-well. This ensures equal incubation times for all wells.
• Do not mix or interchange different reagent lots from various kit lots.
• Do not use reagents after the kit expiration date.
• Read absorbance immediately after adding the stop solution.
• Incomplete washing will adversely affect the test outcome. All washing must be performed with Wash Solution provided. All residual wash liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
• Because TMB is light sensitive, avoid prolonged exposure to light. Also avoid contact between TMB and metal, otherwise color may develop.

Stock: 4 °C

Date de péremption: six months

Détail du antigène

Antigène: HBsAg PreS1 (HBV PreS1) (Hepatitis B Surface Antigen PreS1 (HBV PreS1))

Classe de substances: Viral Protein